Optimization of large-scale production process for human prothrombin complex concentrates / 中国输血杂志

【Objective】 To determine the optimal process conditions for efficiently extracting human prothrombin complex concentrates from human plasma. 【Methods】 Using human plasma as the materials and the yield of prothrombin complex concentrates as the evaluation standard, the preparation process parameters were studied and optimized through design of exporement(DOE), orthogonal experiments, and single factor experiments. 【Results】 The optimal process conditions were as follows: DEAE Sephadex A50 gel was selected, which balanced to pH 7.6, and then the amount of 1.7-2.5 g/L of plasma weight is added into the cryoprecipitate supernatant for adsorption for 40 minutes; Washing solution (0.15-0.175 mol/L sodium chloride) with 3 times the volume of gel was washed 3 times, and eluent (0.5-2.0 mol/L sodium chloride) was washed 3 to 5 times; Add stabilizer (heparin 35 IU, sodium chloride 0.1 mol/L) for ultrafiltration dialysis. 【Conclusion】 By using the optimized process mentioned above, the yield(measured by human coagulation factor IX)can reach 620 000 to 630 000 IU/ton of plasma, which is suitable for large-scale production.

Chiral fluorescence recognition of glutamine enantiomers by a modified Zr-based MOF based on solvent-assisted ligand incorporation.

In this study, three types of chiral fluorescent zirconium-based metal-organic framework materials were synthesized using l-dibenzoyl tartaric acid as the chiral modifier by the solvent-assisted ligand incorporation method, which was the porous coordination network yellow material, denoted as PCN-128Y. PCN-128Y-1 and PCN-128Y-2 featured unique chiral selectivity for the Gln enantiomers amongst seven acids and the highly stable luminescence property, which were caused by the heterochiral interaction and aggregation-induced emission. Furthermore, a rapid fluorescence method for the chiral detection of glutamine (Gln) enantiomers was developed. The homochiral crystals of PCN-128Y-1 displayed enantiodiscrimination in the quenching by d-Gln such that the ratio of enantioselectivity was 2.0 in 30 seconds at pH 7.0, according to the Stern-Volmer quenching plots. The detection limits of d- and l-Gln were 6.6 × 10-4 mol L-1 and 3.3 × 10-4 mol L-1, respectively. Finally, both the maximum adsorption capacities of PCN-128Y-1 for the Gln enantiomers (Q e(l-Gln) = 967 mg g-1; Q e(d-Gln) = 1607 mg g-1) and the enantiomeric excess value (6.2%) manifested that PCN-128Y-1 had strong adsorption capacity for the Gln enantiomers and higher affinity for d-Gln.

Effect of early rehabilitation physiotherapy on muscle quality and function in critically ill patients / 中华危重病急救医学

Objective To investigate the effect of early rehabilitation physiotherapy on muscle quality and function in critical patients. Methods A prospective randomized controlled study was performed. Adult critically ill patients admitted to intensive care unit (ICU) of Anhui Provincial Hospital from October 1st, 2016 to August 31st, 2017 who had been hospitalized for more than 7 days and had acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ ) > 8 were enrolled, and they were divided into treatment group and control group according to random number table method. All patients were given routine treatment, and on this basis, the treatment group was given rehabilitation therapy within 24 hours after admission, including limb active / passive activities, respiratory muscle function training and transcutaneous electrical nerve stimulation, etc. Bedside ultrasound was used to measure the area and cross sectional thickness of left rectus femoris muscle and the cross sectional thickness of middle thigh muscle of patients at 1, 4 and 7 days after treatment; at the same time, the muscle strength of sober patients was evaluated by medical research council (MRC) muscle strength evaluation method, and the mechanical ventilation time, ICU hospitalization time and ICU expenses were recorded. Results Forty patients were enrolled in this study, with 20 in each group. Compared with the control group, the difference of left rectus femoris muscle area between 1 day and 4 days, 4 days and 7 days, 1 day and 7 days (cm2: 0.19±0.02 vs. 0.31±0.19, 0.02±0.01 vs. 0.08±0.05, 0.04±0.02 vs. 0.38±0.23), and the difference in left rectus femoris thickness (cm: 0.01±0.01 vs. 0.14±0.13, 0.03±0.03 vs. 0.16±0.14) and the difference in middle thigh muscle thickness (cm: 0.02±0.02 vs. 0.11±0.09, 0.03±0.02 vs. 0.16±0.12) between 1 day and 4 days, 1 day and 7 days in the treatment group were significantly reduced (all P <0.01). The MRC strength score in the treatment group was significantly higher than that of the control group at 7 days (52.06±3.52 vs. 47.94±3.96, P < 0.05). The mechanical ventilation time in the treatment group (n = 15) and the control group (n = 13) were (138.5±34.5) hours and (185.0±40.9) hours, respectively, and the difference between two groups were statistical significance (P < 0.05). Compared with the control group, the incidence rate of ICU acquired muscle weakness (ICUAW) in the treatment group was significantly decreased [5.0% (1/20) vs. 40.0% (8/20), P < 0.05], the length of ICU stay was significantly shortened (days: 17.67±4.91 vs. 22.06±5.94, P < 0.05), and the ICU expenses were significantly reduced (ten thousand yuan: 7.53±2.09 vs. 9.55±1.73, P < 0.05). Conclusion Early rehabilitation physiotherapy can improve the muscle quality and function in critical patients, and decrease the length of ICU stay.

Semantic type framework in top-level ontology of military medicine / 军事医学

Objective To analyze the use of the semantic type of the top-level ontology of military medicine in order to find problems with the semantic type framework and make it more scientific and comprehensive.Methods Using data driven method, the frequency with which the semantic type of the top-level ontology of military medicine was used in military medical core concepts was statistically analyzed.Results By ranking the use frequency of the semantic types, the semantic types which had higher or lower frequency, and those that had never been used were analyzed.Conclusion The established semantic type framework of the top-level ontology of military medicine is basically consistent with the expression of military medical knowledge and can be constantly updated and improved with the development of military medicine.

CSDC: a nationwide screening platform for stroke control and prevention in China.

As a leading cause of severe disability and death, stroke places an enormous burden on Chinese society. A nationwide stroke screening platform called CSDC (China Stoke Data Center) has been built to support the national stroke prevention program and stroke clinical research since 2011. This platform is composed of a data integration system and a big data analysis system. The data integration system is used to collect information on risk factors, diagnosis history, treatment, and sociodemographic characteristics and stroke patients' EMR. The big data analysis system support decision making of stroke control and prevention, clinical evaluation and research. In this paper, the design and implementation of CSDC are illustrated, and some application results are presented. This platform is expected to provide rich data and powerful tool support for stroke control and prevention in China.

Construction of semantic type framework in top-level ontology of military medicine / 军事医学

Objective To explore the construction method of semantic types of military medical ontology and to establish a framework for the semantic types of the top-level ontology of military medicine in order to lay the foundation for the construction of military medical ontology and provide support for knowledge organization, knowledge disclosure and knowledge service of military medical information resources.Methods Using the method of document measurement, the core concept set of military medicine was constructed.On the basis of the framework of the top-level semantic types of Unified Medical Language System(UMLS), the semantic type framework was completed by the combination of bottom-up and top-down modes, using semantic analysis and expert consultation.Results By classifying the same kind of knowledge points of military medicine into one semantic type, the hierarchical structure of the semantic type framework of the top-level ontology of military medicine was obtained.Conclusion The proposed method is effective and practical for the construction of the semantic types of military medical ontology.The established semantic type framework of the top-level ontology of military medicine could reconstruct military medical knowledge and provide the basic framework for the establishment of the semantic relationships of the top-level ontology of military medicine.

Effect of systemic chemotherapy on conditions of tumor infiltrating, metastasis and disease-specific survival for advanced retinoblastoma / 中华眼底病杂志

Objective To observe the effect of systemic chemotherapy on conditions of tumor infiltrating,metastasis and disease-specific survival (DSS) for advanced retinoblastoma (RB).Methods Forty-one patients with advanced RB who received enucleation were enrolled in this study.There were 26 males and 15 females,age at diagnosis was ranged from 2 to 72 months,with a mean of 23.08 months.There were 16 bilateral patients and 25 unilateral patients;13 group D eyes and 28 group E eyes.16 patients received enucleation as the primary treatment (operation group),25 eyes received chemotherapy before enucleation (chemotherapy group).There was no significant statistical difference between two groups for the gender,unilateral and bilateral,international staging or diagnostic age (P＞0.05).The histopathology report was performed to assess the risk of postoperative tumor-node-metastasis staging (pTNM) in each patient,and the extent of tumor invasion in the optic nerve,choroid and anterior chamber was divided into 3 levels of low risk,medium risk and high risk.Five deaths were all in the group E with chemotherapy before enucleation.Using R software survival analysis software package survfit function,the application of Kaplan-Meier estimation method,DSS of RB children was calculated from the time of diagnosis,up to the date of the death of patient.DSS differences between chemotherapy,operation group and eye removal time (more than 3 months,less than 3 months) in group E RB children were analyzed.Results The proportion of high risk pTNM stage in chemotherapy group was significantly lower than the operation group.But there was no significant difference between the two groups in the overall risk classification (x2 =3.130,P=0.077).For group D eyes,the overall risk classification in chemotherapy group was significantly lower than the operation group (x2 =5.870,P=0.015).There was no significant difference between the two groups in the overall risk of group E eyes (x2 =0.020,P=0.889).The DSS in chemotherapy group and operation group were 0.71 and 1.00,respectively;the difference was significant (x2 =3.700,P=0.05).The DSS in children whose enucleation delayed for more than 3 months and children whose enucleation performed within 3 months were 0.64 and 1.00,respectively;the difference was significant (x2 =4.800,P=0.028).Conclusion Systemic chemotherapy did not reduce the risk of tumor invasion and metastasis in patients with advanced RB.Instead,it will reduce the DSS in group E eyes of RB.

Application of Docker technology in bioinformatics / 军事医学

With the rapid development of biotechnology such as NGS and proteomics , bioinformatics has seen an explo-sion in diversity and complexity in terms of data , tools and demands .The traditional computing environment , including ded-icated workstations and virtual machines , are no longer suitable under such circumstances .As a rising container technolo-gy, Docker, which is characterized by light weight , openness and security ,has provided an innovative solution to analysis and processing of biological big data and attracted increasing attention from bioinformatics developers and users .Consider-ing the demands and features of development , deployment and application of bioinformatics tools in the age of big data , this paper analyzes the advantages of Docker in this field , introduces some actual cases and discusses current deficiencies and future improvement .

Agent-based modeling of medical evacuation of the wounded during earthquake relief / 军事医学

Objective To study and construct related models of medical evacuation of the wounded during earthquake relief.Methods Based on the Agent model,the boundary of the medical evacuation system for the wounded was determined and the Agent model system was established according to the medical service theory about medical evacuation of the wounded during earthquake relief.Using Agent-based modeling,the structure,attribute,related behavior and function of key Agent models were analyzed and the model structure was presented.Results and Conclusion The Agent model system including 11 Agent models is divided into business Agent modeling,assistant Agent modeling and environment Agent modeling.Six key models are built,and the interaction and coordination mechanisms of various types Agent models are established.

Kmer-indexer: A Fast K-mer Indexing Program.

In our previous work, a k-mer based program named MFEprimer-2.0 was designed and developed for primer specificity evaluation task. In order to increase the index processing speed, decrease the index data size and memory usage, we developed a new program named kmer-indexer. Compared to MFEprimer-2.0, the processing speed of kmer-indexer is 10+ times faster, the index data size can be significantly reduced to one third, and the memory usage is controlled within a small range so that this program can run on an ordinary PC.

Keys to the information security and privacy protection of electronic health records / 中华医学图书情报杂志

The information security and privacy protection of electronic health records (HER) are greatly con-cerned by healthcare administrators, healthcare providers and patients in the era of big data and cloud computing. The concepts of information security and privacy protection of HER wre defined according to the systematic investi-gation of related literature, the keys to the information security and privacy protection of HER were summarized, and the advances in research on information security and privacy protection of HER were described.

Informationization of military medicine / 军事医学

Military medicine has to restructure itself to meet military needs and e-Science patterns of the information age.This restructuration is known asinformationization of military medicine ( IMM).IMM is a process of vision refresh-ing into information thinking, mission expansion to the information field and paradigm shift to normal forms of information. The result is the emergence of military med-information technologies, military med-information research fields, and military med-informatics disciplines.

The value of hardness rating and area ratio method of ultrasound elastography in differential diagnosis of benign and malignant lesions / 中国基层医药

Objective To compare the value of hardness rating and area ratio method of ultrasound elastog-raphy in differential diagnosis of benign and malignant lesions.Methods ultrasound imaging data of 120 cases with breast lesions(130 breast)who were pathologically confirmed during the period from January 201 1 to June 2013 were retrospectively analyzed,there were 62 malignant tumors,68 benign tumors,the sensitivity,specificity,accuracy of hardness rating and area ratio method of ultrasound elastography in differential diagnosis of benign and malignant lesions were assessed.Results (1 )The ultrasound elastography of 52 cases with benign tumor were 0 -2point, 16 cases were 3-4point,10 cases with malignant tumors were 0 -2point,52 cases were 3 -4point,the difference between two groups were statistically significant(χ2 =11.55,P<0.05);(2)The average area ratio of benign tumor was (1.09 ±0.37),malignant tumors was (2.28 ±1.68),the difference between two groups were statistically signifi-cant(t =6.22,P=0.01);(3)the sensitivity,specificity,accuracy of elastography hardness rating in diagnosis of malignant breast tumors were 83.87%,6.47%,80.00%,the area ratio method were 80.65%,75.00%,77.68%, joint inspection of two groups were 93.10%,93.55%,93.09%,joint inspection had obvious advantages,the differ-ence was statistically significant(all P<0.05 ).Conclusion Hardness rating and area ratio method of ultrasound elastography in differential diagnosis of benign and malignant lesions had higher value and higher diagnostic accuracy of combined.

Comparison of efficacy of ultrasound -guided percutaneous catheter drainage and incision and drainage catheter in treatment of liver abscess / 中国基层医药

Objective To compare the efficacy of ultrasound -guided percutaneous catheter drainage and incision and drainage catheter in treatment of liver abscess.Methods 50 cases with liver abscess who had given ultrasound -guided percutaneous catheter drainage as the observation group were randomly selected and 50 cases with liver abscess who had given open catheter drainage over the same period were selected as the control group.The cure rate,white blood cell count(WBC),body temperature returned to normal,extubation time,hospitalization and the occurrence of complications of two groups were compared.Results (1)The WBC,body temperature returned to nor-mal,extubation time,hospitalization of the observation group were (4.35 ±1.45 )d,(3.68 ±1.68)d,(10.85 ± 1.66)d,(13.76 ±2.80)d,which were significantly shorter than (7.66 ±2.83)d,(6.22 ±2.75 )d,(13.05 ± 1.95)d,(17.96 ±2.91 )d of the control group.The difference between the two groups had statistically significant (t1 =8.352,t2 =9.145,t3 =7.552,t4 =8.443,all P <0.05);(2)One case of postoperative wound infection of the observation group,the complication rate was 2%,two cases of postoperative wound infection of the control group,one case of empyema,two cases of bleeding,the incidence was 10%,the difference in the incidence of complications between the two groups were statistically significant(χ2 =6.73,P <0.05).Conclusion Ultrasound -guided percu-taneous catheter drainage was trauma,quick recovery,safer,which is worthy of clinical application.

A cloud computing-based implementation of regional medical information sharing / 军事医学

Objective To establish a regional medical information sharing platform used to promote the sharing of regional medical data such as electronic medical records( EMR) and medical images in order to improve medical services. Methods With reference to results and experience of the domestic and foreign research and by virtualizing re-establish-ment of the existing medical information resources,a regional medical information sharing platform was established based on cloud computing, SOA,and Web services, and then the heterogeneous information systems in different medical institutions were integrated.Results A novel cloud platform solution was proposed for clinical information exchange between medical institutions based on Web services that exchanged data and ensured the data security and privacy using XML encryption and compression.The prototype developed in a cloud environment was put into use in dozens of military hospitals to enable the sharing of clinical information.Conclusion This cloud computing platform has the advantages of dynamic scalability, distributed and ubiquitous access.It is highly feasible and practical for regional medical informatization.

Progress in algorithms of microorganism detection based on high throughput sequencing data / 军事医学

The development of high throughput sequencing technology has promoted the development of many related spheres of research .Many research institutions are studying algorithms of microorganism detection using high throughput se -quencing data .Some algorithms have already been designed and published .In this paper , we investigated the literature re-garding the use of high throughput sequencing data for microorganism detection , studied the function and workflow of four released algorithms of microorganism detection based on high throughput sequencing data , and analyzed their advantages and disadvantages.Finally, we summarized how these detection algorithms were designed and classified them before we proposed ways of improvement .

Design and implementation of a military disease prevention and control website / 军事医学

Objective To establish a military disease prevention and control website to achieve dissemination of disease control information , resource sharing , business communication in the whole army , and popularize relevant knowledge of disease prevention and control promotion among the troops .Methods Based on the content management system and meta-data repository platform,B/S system architecture, JSP,MySQL and other programming techniques and tools were used to complete the system development .Results The military disease prevention and control website we designed has been for-mally launched ,which was able to implement a set of functions such as content distribution , full-text retrieval ,classification and aggregation of knowledge , online consultation .Conclusion This website plays a positive role in enhancing military prevention and control ,popularizing relevant laws and regulations ,meeting the health needs of the army soldiers and impro-ving the ability of disease prevention in grass-roots units.

Analysis on the core connotation of ability health management for military personnel / 军事医学

In this paper, we generalized the basic concept of ability health management and discussed its core connota -tion in terms of ability health measurement , ability health assessment and ability training .After defining ability health meas-urement, outlined the process of determining the measurement items and listed the main contents of measurement standardi -zation.Further, we systematically analyzed the basic components of ability health assessment , including the index system , standards, methods and process .We also discussed the function , classification and principle of ability training .Finally, we drew the conclusion that the goal of ability health management is to improve the ability health level so as to consolidate and promote the operation capability of military personnel .

Design and implementation of an Internet information dynamic tracking system for infectious disease surveillance / 军事医学

Objective To analyze the requirements of the Chinese PLA biosecurity and disease prevention and to develop an Internet information tracking system for infectious disease surveillance .Methods A focused crawler was used to automatically collect Internet information about infectious diseases before the information could be released , automatically correlated and analyzed .Results This system could claw the Internet information of infectious disease surveillance and build an infectious disease surveillance information knowledge database .Conclusion The use of this system can not only improve the efficiency of information studies , but also meet the information requirements of infectious disease prevention in Chinese PLA.

Construction of a three-dimension model on evaluation of human resources for military medical sciences / 军事医学

Based on a correct understanding of the related concepts and system of the evaluation of human resources for military medical sciences ( EHRMMS), this thesis established a three-dimension model of EHRMMS that is composed of the evaluation goal, evaluation subject and evaluation object, and analyzed the factors that affect the crucial parameters in the model.We also analyzed the features and regularity of the evaluation model, and tentatively explored the application of the model.